FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13086256
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA459270.35095599535879474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA3550.026.4316724
TATGCCG6350.024.66072848
CACCTAT8800.023.7074681
GCGAATA7750.022.97825659
CGTATTA1403.5265475E-722.3401158
CGCGAAT7800.021.47765458
AACGTTA3150.021.05151258
CGAATAT8250.020.75037460
TACGTAT3200.020.6341323
CGTACGC46250.020.2166585
GTTACGT7850.019.9256135
CCGTACG47200.019.8833984
ACGCGAA3350.019.7157938
AATACGT5850.019.6107139
TTCGGCG48000.019.56247544
CTCGCCG68350.019.48503170
ATACGTA5900.019.4448940
AACCTAC5200.019.38786339
TACGTAA5950.019.2827441
CGCAACG4300.018.93979570
Produced by FastQC (version 0.11.5)