FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14517156
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT171600.11820497072567106No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT146470.10089441761182424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12800.038.33042548
ATAATAC39450.034.080417
GATCGGA16550.033.9341931
TAATACA41750.032.1199218
AATAATA43950.030.98515116
TATTGAA49950.028.7202229
GATTTAT49350.028.5117035
CGGAACG24350.028.29441348
GAAAATA49600.027.7350413
TCGTATG18100.027.458545
GTCCGTT16350.027.1401821
CGACTTA6100.026.83226867
AATTCGT20850.026.44325435
GCACTAC52050.025.56515125
TACGACA12700.025.3747759
ACCGCGA22850.024.95445846
AGTCACG22500.024.79632228
GTACGGT8000.024.6811870
ACCGCAT52800.024.53030254
TGTTACG8800.024.4172233
Produced by FastQC (version 0.11.5)