FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14517156
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT150380.1035877826207833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA13150.026.0312589
TAGCTCT47400.025.79145840
CTCGATT47250.025.51055549
ATAGCTC47100.025.51033439
TCTCGAT47700.025.40330748
TTCTCGA51200.023.72503947
ATTCCGT51300.023.54410753
TGATAGC54650.022.54778937
TTGATAG56150.022.00230636
CGGACAC58750.020.9532713
ACACGGA57950.020.94432416
TTCCGTG58050.020.88080454
GGATTGA62250.020.16512725
CCGGACA64000.019.9908612
CGTGGGT61000.019.79970257
TTTCTCG63150.019.66734546
ACGGACA63300.019.44654718
CACCCGG64950.019.1067034
CGGACAG65100.019.06826219
ACCCGGC64900.018.963075
Produced by FastQC (version 0.11.5)