FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17499026
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT473760.27073506834037503No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT354480.20257127453836574No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG196160.11209766760732856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190970.10913178824924312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA46950.034.5981839
GCATGGC181550.033.93704270
ACACGGA116800.032.44378316
CGGACAC118700.031.63219613
ACGGACA128350.029.87394718
CGGACAG131000.029.53386319
TCACCCG129200.029.375373
CCGGACA129500.029.18095812
CACCCGG131650.028.8286974
ATAGCTC132000.028.73165939
TCTCGAT131400.028.72915548
CCCGGAC132100.028.60653511
ACCCGGC133600.028.4868165
TTCTCGA133300.028.41621647
TAGCTCT134800.028.13896640
GGATTGA137200.028.12559525
CCAGTAG58300.028.0366485
CTCGATT134750.028.02352149
TTGATAG137650.027.81846236
CACGGAC139350.027.41727417
Produced by FastQC (version 0.11.5)