FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15074740
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211090.1400289490896692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG21150.017.22960315
ACGCACT21100.015.66943370
TCTCGAT26150.015.53349248
TCGCGAC10650.015.31480334
CTCGCGA10800.015.10234933
CTCGATT26550.015.03923649
CGTAGAT51100.014.6761071
GTGTCGT52150.014.24150314
ATGCGGT51550.014.0738246
GGCGCGT17400.013.9590926
TTCTCGA30600.013.84085947
TAGATCG29350.013.7142717
GGTGTCG83750.013.30194413
AATGCGG57100.013.19156645
GTAGATT61850.013.1853842
TAACGCG4254.5474735E-1013.06476133
TAGATTC63850.013.0417893
TCGTGGC80500.012.80871770
GTCGTGA59300.012.75828716
CATTGTC101950.012.75218170
Produced by FastQC (version 0.11.5)