FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14486318
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT222300.15345514298388313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14150.037.5963821
TATGCCG14300.035.76287548
ATAATAC58050.031.72902717
GCGCGTT8800.031.72240662
TAATACA59400.030.77410518
AATAATA62100.029.60388216
GATTTAT73950.026.3217015
CTCGCCG50750.026.14107770
TCGTATG20750.025.97880445
GCACTAC71850.025.05694425
GAAAATA73850.024.98757613
TATTGAA76700.024.6483679
AGATTTA71750.024.319014
ACGTTAA9500.023.41189213
ATTGAAA80500.022.96687310
AGCACTA80700.022.52370324
CGATACG11350.022.3727647
GTCACGA24050.022.25554529
GACGTTA10200.022.14597312
CACTACA82200.022.11352326
Produced by FastQC (version 0.11.5)