FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15467746
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAT43200.020.8838961
GCTACCG33900.019.6630670
TTATCGT17650.018.7873336
TTGCCGA42850.018.74851669
ACGCACT28300.018.11844870
TAGATTC52600.017.9918273
TACGTAT4650.017.91119448
TACGCCA41800.017.77019123
ATTGCCG40550.017.58433368
GTAGATT53500.017.4352112
ATGCGGT50800.017.3341246
TGTACGC41300.017.2322321
TCTCGAT20550.017.05594648
TGCCGAC65150.016.87748770
GTTATCG19800.016.7461555
CCATGTA43700.016.3436854
GTACGCC43550.016.34219622
CCTAAAT50900.016.33490444
TCACGAG38500.016.2396817
CTAAATT51550.016.0671545
Produced by FastQC (version 0.11.5)