FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17786882
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT283070.15914537466431722No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA275150.15469265495773796No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG190630.10717448960419257No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG189200.10637052632383798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19450.037.0665551
ATAATAC66350.035.65808517
TAATACA69250.034.26515618
TATGCCG17250.033.49963848
AATAATA73250.032.72459416
TGTTACG18600.032.0400163
GCACTAC74050.031.76480325
GCGCGTT8650.031.29885362
TATTGAA82050.029.8052929
GATTTAT82600.029.7819465
GAAAATA81500.029.28381313
AGCACTA82750.028.63385224
ACGAATT25250.027.58554332
CGGAACG48950.027.237548
CACTACA87650.026.9944926
TACGACA12500.026.8799829
ACTACAG90250.026.63919327
GTTACGA22900.026.477524
TCGTATG22500.025.95705445
AATTCGT26800.025.87245635
Produced by FastQC (version 0.11.5)