FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17786882
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT477330.2683606941340253No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT216670.12181449227582439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA37500.036.322819
CGGACAC110450.031.30349213
GCATGGC185900.030.75181470
ACACGGA113800.030.50377816
ACGGACA118400.029.784318
CGGACAG118700.029.68039719
CACCCGG116700.029.558514
CCGGACA118600.029.38558612
TCACCCG120300.029.1632213
CCCGGAC122050.028.3849711
ACCCGGC126050.027.175615
CACGGAC130150.026.99031817
ATAGCTC130100.026.75029839
TCTCGAT130050.026.6612248
CCAGTAG51100.026.5839885
TAGCTCT131500.026.41916740
GGATTGA134200.026.33095725
TTCTCGA132250.026.23292447
CTCGATT132400.026.14676549
GCCCGGA134350.025.96531910
Produced by FastQC (version 0.11.5)