FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15502712
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT6500.020.7429770
CGTAGAT57650.019.0085851
TAGATTC64400.017.9737243
GTAGATT65650.017.7398222
CTAAATT53250.017.4577445
TTATCGT25850.017.4288486
GTTATCG28950.016.8787785
TAAATTC57450.016.48579846
CGCCTAA18250.016.16735649
CCTAAAT58600.015.92181144
CGCATTT36400.015.91066643
TATCGTG29450.015.8875447
ATGCGGT69250.015.53027946
TAGCGAT3603.274181E-1115.40541630
AATGCGG71900.015.19688245
TAAATGC73450.015.10885243
GTCTCGC42700.015.0151536
TATAAAT73750.014.95141541
GTGTCGT32900.014.85594414
AAATGCG75350.014.77609244
Produced by FastQC (version 0.11.5)