FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15399940
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT181500.11785760204260536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156330.10151338251967217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCG40750.026.8977570
TAGCATA21050.026.8624179
TCTCGAT54000.026.22877748
TTCTCGA56450.025.39599847
CTCGATT56000.024.67405749
ATAGCTC57500.024.55996939
ACCCGGC57150.024.4587065
ACACGGA56550.024.41661316
TAGCTCT58000.024.34848640
ATTCCGT58650.023.39317953
CGGACAC60150.023.0711513
ACGGACA61250.022.88203818
TTCCGTG62150.022.07923354
CGGACAG64300.022.06655919
CGTGGGT62050.022.01301257
GCATGGC103150.021.96062970
CACCCGG63250.021.9341874
TGATAGC67850.021.42657537
CCGGACA65800.021.3540212
TCACCCG67950.021.1834283
Produced by FastQC (version 0.11.5)