FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18841103
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT792330.420532704481261No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG375750.19943099934223596No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG236300.12541728581389316No Hit
CGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCG209360.11111875987302867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGGC245650.039.15465570
TAGCATA78100.038.4421469
TCACCCG157450.038.1082533
ACACGGA158900.037.81347716
ACCCGGC162050.036.790235
CGGACAC163000.036.69197513
CACCCGG165650.036.0519264
CCGGACA172900.034.89128512
ACGGACA174500.034.7695918
GCCCGGA174900.034.27342210
CGGACAG176650.034.20938519
CCCGGAC175850.034.1284211
GACACGG178100.033.98999415
CACGGAC179700.033.66779317
CCAGTAG89650.033.2894025
CTCACCC181750.033.154692
GTAGCAT91100.033.108318
GGACACG188750.032.20081314
CCTCACC196600.031.0026631
GGATTGA207100.029.2982325
Produced by FastQC (version 0.11.5)