FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14681452
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT299720.2041487449606483No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA253790.1728643733603461No Hit
CCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT161870.11025476226738337No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG149830.10205393853414499No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGT147760.10064399624778257No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG147400.10039878889363259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC68650.035.7386417
GCGCGTT9600.035.37623662
GCACTAC67800.035.31970225
TAATACA71050.034.48258218
CGGAACG31600.034.12892548
AATAATA75100.032.71541616
AGCACTA78050.030.94756924
GATTTAT83150.030.8497965
TATTGAA82500.030.5805669
CACTACA79150.030.51819826
ACTACAG81200.030.3891527
ACCGCGA17450.030.28945746
GAAAATA81250.030.0682713
TGTTACG12950.029.216313
CGCGACG18100.029.21547948
GTCCCCG83650.028.74628449
CTCCCGT70000.028.4736961
CGCTCGC39800.027.7033656
CTACAGA87850.027.69362328
GCGACGC19900.027.27979749
Produced by FastQC (version 0.11.5)