FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14681452
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT346420.23595758784621576No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT152250.10370227685926434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC83800.033.9322913
ACCCGGC83900.033.800525
GCATGGC135900.033.78454670
ACACGGA86000.033.0238716
TAGCATA26400.032.7955259
TCACCCG89350.031.9312863
CACCCGG89400.031.680834
ACGGACA90200.031.63889918
CCGGACA92500.031.0774912
CCCGGAC91800.030.93718111
CGGACAG94000.030.61789719
CACGGAC96350.029.65604817
TCTCGAT95550.029.64998248
ATAGCTC97200.029.16726939
CTCGATT97250.029.06852149
TTCTCGA98650.028.78077547
TAGCTCT100300.028.44195740
GCCCGGA99800.028.42138110
ATTCCGT100250.027.78495653
GACACGG105500.026.88700915
Produced by FastQC (version 0.11.5)