FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14936090
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT268460.17973914190393872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196180.13134628942380502No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG169960.11379149429335254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA44250.031.209989
ACACGGA76600.028.7218116
CGGACAC80750.026.94470413
ACCCGGC79950.026.9040935
GCATGGC134250.026.41441570
ACGGACA85100.026.13783518
CACCCGG84750.025.2152274
CGGACAG87850.025.16137919
CCAGTAG55150.025.0370245
CCGGACA87350.024.9485412
TCACCCG87400.024.9274223
CCCGGAC88600.024.40064411
CACGGAC92600.023.94621517
GTAGCAT58750.023.506548
GACACGG96400.022.96666115
TCTCGAT96350.022.34480548
CTCGATT98050.021.92366449
GCCCGGA101400.021.66219910
TAGCTCT100800.021.62423140
TTCTCGA99300.021.50412247
Produced by FastQC (version 0.11.5)