FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17248368
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCTTCTTCAGTCACTTCTTCAGTCACTTGGGTCTCCTCTGTCTGTTCCTCCACAATCACAGTTTCTTTCTC332540.19279505168257077No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG284620.16501271308682652No Hit
CTCCGGAGTCGCAGTGTCTTCGGCCGCCGGAAGGTGGGTGACGTGCGGAT268700.15578285435468445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA258090.14963154774990886No Hit
CTCCAGTCTTCAGTAGGGAATTGCTGAATAGGCACAGAGGGCACCTGTACACCTTCAGACCAGTCTGCAACCTC220990.1281222664080451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG655.4226985E-737.1838826
AGTCGCA59750.035.4217457
TACGACA13250.035.177429
CGAATTA5350.034.22038334
AATAGGC61600.033.9115227
AATTATA5950.033.1048836
AATAATA17200.032.1181316
ATAATAC17350.031.64126217
AACGTTA7100.031.45836361
TGAATAG66800.031.3747125
TTTAACG4500.030.82410644
CCGGAGT76400.030.5111473
GCGCGTT18000.030.39288962
TAACGGG4500.030.09654246
GTACTAA18850.029.85545212
ACGTGCG70050.029.70395541
TTACGAC16450.029.383828
TACGTAA6000.029.3540695
CGTTAGA7650.029.32009763
TGACGTG71650.029.25859339
Produced by FastQC (version 0.11.5)