FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17939994
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGATTCGGGCAAGTCCACCACTACTGGCCATCTGATCTATAAATGCGGTGGCATCGACAAAAGAACCATTGA208760.11636570224048014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCG43150.027.23779570
TCTCGAT35150.020.83149548
CGTAATT6050.020.74688370
CGCCTAA20450.020.7081649
CTCGATT36050.020.41294149
TTCTCGA36550.020.12379547
CGTAGAT92900.019.65951
ATGCGGT90750.019.14946646
ATAGCTC39800.018.9810739
TAGCTCT40600.018.60877240
TATCGCT23150.018.56041538
GTATCGC23400.018.36058237
AATGCGG94650.018.28304145
ATTCCGT40000.018.07050353
TAGATTC108550.017.833283
AAATGCG99800.017.44080544
GTAGATT109800.017.2239512
ACGTATA5650.017.16532716
TCGCGAC11200.017.01231834
GCATCGA108600.016.38979554
Produced by FastQC (version 0.11.5)