FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005293673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15450745
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT859740.5564391878838204No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGT449700.29105392652587303No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA364170.23569737252151918No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG307840.19923958359289473No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249950.1617721346122792No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC239430.15496340144116028No Hit
CCCTGACTTTCTTCATTCTGTTCTTGCGTTCCTTTCGTTGCTTTCTTGAG235140.15218683629818497No Hit
GTCCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAG222560.14404483408405228No Hit
CTCTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTACTGGGCCTCTCTGAATTCGCTTCATC215630.13955961346847678No Hit
CTCTGCATCTTCATCTTGGTCACCACGCCAGAGAGGATCCGCCCTCGAAT189540.12267369631690898No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG184170.11919813575332451No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA181090.1172047043686243No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA179610.1162468217551969No Hit
CTCCTCTTCTTCAGTCACTTCTTCAGTCACTTGGGTCTCCTCTGTCTGTTCCTCCACAATCACAGTTTCTTTCTC168090.10879087060203245No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCT164370.1063832197088231No Hit
CTCCTCTTTGCTGATTCTGTGTTTGGCCCCAATGCAGCCTGTCCTGCGCT162220.10499170104742521No Hit
CTCCCCATAGATGGACTTGCCACCAGTGCCATTATGGCGTGTGAAGTCACCACCCTGACACATAAACCCTGGAAT160860.1041114845918433No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG157100.10167794497935212No Hit
CTCCTTTCTCTCTCCTCCTCCTGCAGCTTGATGGAGATACCTCTTACTGGGCCTCTCTGAATTCGCTTCATC155600.10070711800628383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)