FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15450745
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT416330.2694562624650138No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT205470.1329838787708942No Hit
CGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCG188230.12182584076042935No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG177620.11495885797092632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGGC133550.038.06382870
TAGCATA33000.037.8062749
CGGACAC85600.036.07531713
ACACGGA87100.035.5329416
TCACCCG88250.035.0600243
ACCCGGC88200.035.001795
CACCCGG89800.034.4537544
ACGGACA93850.033.7886318
CCGGACA93600.033.213712
CCCGGAC93500.032.8043111
GCCCGGA95100.032.5791210
CGGACAG97450.032.3632219
CACGGAC100400.031.37799517
GTAGCAT43200.028.9587968
TCTCGAT108600.028.76775448
CTCGATT108600.028.67651449
CTCACCC108750.028.5834472
GACACGG109650.028.32080815
ATAGCTC111100.028.15267439
TTCTCGA110700.027.96807747
Produced by FastQC (version 0.11.5)