FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14081183
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG162570.11545194746776602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT5700.030.4994266
ATAATAC34650.029.7963717
TATGCCG7400.028.67415248
AATAATA38200.027.84644516
TAATACA38750.026.64400518
GCGACGC13950.024.4350621
CTCGCCG78050.023.64266870
GATTTAT50000.021.348385
GAAAATA49350.021.13217713
TATTGAA55300.020.4929359
TCGCTAC4250.020.48478755
TCTACGC3600.020.28226340
GATCGGA13950.020.1963271
GACGTTA11550.020.1651512
CTCCCTA11400.019.8321631
CGTACGA29150.019.67845526
CCGACTA4900.019.25662865
GCACTAC53350.019.09320425
AAATAAT56550.018.81044215
ACGTTAA12800.018.73912413
Produced by FastQC (version 0.11.5)