FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14081183
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168120.11939337767288445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG3900.025.796098
ACGTATT7650.021.84768570
GTAACGT5500.020.1859615
ATTAGCG2900.019.41846567
AACGCGC11300.019.3430215
TCGCATA5450.019.1621661
GTAGCGT3250.018.96569668
ATACGTC4950.018.92366830
TAATTCG17450.018.68936515
AGTAGCG5450.017.89106262
CGGTACG3300.017.8712738
CGTAACG4300.017.75073814
TACGTAT6900.017.28183669
CGATAGG2501.8364517E-816.70261660
CGCGTAG2753.4688128E-916.40091933
TTAGCGT3551.2732926E-1116.34161268
GCGATAA6000.016.1906533
TACGCGC2804.3819455E-916.11457446
ACGTAAC4850.015.73776613
TACGGGT13500.015.41944831
Produced by FastQC (version 0.11.5)