FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16963687
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT327220.1928943866978918No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA218920.1290521335367718No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG172480.1016760094665741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC77150.036.39880817
TAATACA83000.033.70798518
GCACTAC82200.033.32081225
AATAATA89250.031.3865616
GCGCGTT19500.030.50014762
GATTTAT97400.030.478025
CGGAACG32800.030.02493348
CGAGGCG89000.029.96465354
TATTGAA96150.029.8926629
AGCACTA94300.029.22892624
ACTACAG95600.029.19526527
CTCGCCG70200.028.47275770
GAAAATA98350.028.30591213
CACTACA99200.027.92579326
GGCGTTT97100.027.68150357
CGTTTTG99750.026.97025559
CCCCGAG100200.026.58822651
CTCCCGT88250.026.5654541
CTACAGA104700.026.49182128
GTCCCCG103350.025.69737849
Produced by FastQC (version 0.11.5)