FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16963687
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT350820.20680645663881914No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210420.12404143038008188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC94350.029.49146713
ACACGGA94750.029.22050716
GCATGGC156000.028.7248770
ACCCGGC97750.028.140415
TAGCATA47800.028.0530559
TCACCCG98650.027.9885393
ACGGACA103400.027.17722718
CCGGACA104200.027.06928812
CACCCGG102350.026.9755464
CGGACAG104800.026.8477119
TCTCGAT107000.025.96999248
CCCGGAC109100.025.72648411
TTCTCGA109600.025.28462247
CTCGATT110450.025.22758749
ATAGCTC111050.024.9239
CACGGAC112450.024.8980717
ATTCCGT109850.024.60139553
TAGCTCT112850.024.5251240
GACACGG116550.023.81444215
TTCCGTG115350.023.6186654
Produced by FastQC (version 0.11.5)