FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19031105
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT843920.4434424590689821No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG415490.2183215320392589No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG249170.13092776273369308No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT227680.11963572267611366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGGC262650.038.83720470
TCACCCG172450.037.495283
TAGCATA87350.036.4256179
CGGACAC178150.036.36345313
ACACGGA181650.035.64365416
CACCCGG181250.035.6373754
CCGGACA186100.035.0334912
CCCGGAC187700.034.56874511
CGGACAG191000.034.3334119
ACCCGGC187950.034.3126345
ACGGACA191850.034.07256318
GACACGG193050.033.70094715
GCCCGGA195550.033.32155610
CCAGTAG95250.032.8175625
CTCACCC199800.032.751222
GGACACG204750.031.808514
CACGGAC206200.031.6180717
GTAGCAT101550.031.263128
CCTCACC216150.030.4828261
GGATTGA215250.030.45085325
Produced by FastQC (version 0.11.5)