FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14873942
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250120.168159859706324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA21700.022.2059339
ACGCACT28050.021.2217870
TCTCGAT50150.020.91130348
CTCGATT51800.020.11425249
TTCTCGA54600.019.33015347
CGGACAC54400.019.31014313
TAGCGTT7050.018.93882670
TAGCTCT55400.018.84663240
ATAGCTC56050.018.44122939
ACACGGA57200.018.18250816
ATTCCGT57550.018.00068353
ACGGACA59200.017.91857518
GGCGCGT23800.017.7673955
TTCCGTG60250.017.65905254
GTCGAAC11400.017.56825370
CGGACAG61550.017.34719719
ACCCGGC60800.017.3304925
TTGATAG63750.016.64578436
TGATAGC63950.016.48593537
GCATGGC102550.016.4375870
Produced by FastQC (version 0.11.5)