FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16465183
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290060.1761656703117117No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT209950.12751148894002576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC53250.032.60584317
CGGAACG24550.032.60409548
TAATACA57150.030.44153218
GGCCTAA36150.029.91178966
AATAATA61100.028.98513416
GCACTAC59200.028.97837425
GCCTAAG40250.027.621567
GAAAATA64700.027.31877113
CTCCCGT87100.026.7247771
GATTTAT70200.026.4695245
CGCGACG16200.026.4232348
GCGCGTT9550.025.63116562
ACCGCGA16900.025.52561246
CCTAAGT44550.025.39713968
CTAAGTC48700.024.80659769
TATTGAA72850.024.739199
AGCACTA69900.024.59171924
ACTACAG73550.024.41042327
CACTACA72400.024.17475126
ACTTTCG52800.023.6151836
Produced by FastQC (version 0.11.5)