FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21014187
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13629306.485761262141619No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490640.7093493552712745No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA854460.40661101949839884No Hit
CTCCTCTTCGGCAGCTCTCTTCAGCGCGGGCCCTTCATCATCCTCTTCTT560970.2669482288322646No Hit
CTCTTCTTCTTCTTCCTCATCTTCTTCTTCCCCTTCATCTTCCTCCTCTC411270.19571064062578297No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG384030.18274796926476383No Hit
CTCCAGTCTTCAGTAGGGAATTGCTGAATAGGCACAGAGGGCACCTGTACACCTTCAGACCAGTCTGCAACCTC381580.18158209023266045No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA362530.17251678592181557No Hit
CCCATTTTCTGTCTTCTGCCGTTTGGGATCCGCTTCATCCTCCTCTTCGGCAGCTCTCTTCAGCGCGGGCCCTTC334430.15914486722707855No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313650.14925630955887087No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA284000.13514679392545617No Hit
CTCCTCTTCTTCAGTCACTTCTTCAGTCACTTGGGTCTTCTCTGTCTGTTCCTCCACAATCACAGTTTCTTTCTC267990.12752813135240493No Hit
CTCTTCTTCAGTCACTTCTTCAGTCACTTGGGTCTTCTCTGTCTGTTCCT252690.12024733576416731No Hit
CCTCCTCTTCGGCAGCTCTCTTCAGCGCGGGCCCTTCATCATCCTCTTCT243370.11581223675224742No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229260.10909772526531719No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219060.10424386153982546No Hit
CTCTTCGGCAGCTCTCTTCAGCGCGGGCCCTTCATCATCCTCTTCTTCTTCTTCCTCATCTTCTTCTTCCCCTTC213770.10172651456846749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCG30800.061.0945570
CGACTTA21500.058.35980267
CTAAACG6800.057.08417528
ACGTTAG4900.052.71788462
AATAGGC71600.049.5121627
TGAATAG74050.047.9200625
CGTTAGA5450.047.51434363
GACTTAT27100.047.4518168
GTTAATA14300.046.600137
GACTACT14250.046.52535226
ATATGAC14400.046.27673711
ACTTATC28950.045.82622569
TACGCGC2700.045.799447
ATATGTA12850.045.10759739
GAATTGC79850.045.08247818
AACGTTA5800.045.02912561
GATACTA5850.044.64475631
ATATACG1550.044.34156431
CTGAATA80600.044.11073324
TACGTAA3050.043.36979369
Produced by FastQC (version 0.11.5)