FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15285099
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT208300.13627651348545405No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA160350.10490609187418413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAC55050.026.28504825
GACGTTA39900.025.45398112
ATAATAC58550.025.12791317
CGCAATC23950.024.2525114
CGAGGCG57800.024.02517154
ACGTTAA42550.023.78697213
ACTACAG64500.022.86540227
CACTACA64650.022.59721626
CTCGCCG39400.022.3790670
TAATACA68500.022.08724818
AATAATA67500.022.0023216
TAGCGCT46700.021.67675836
TTGGTCG29350.021.0938689
CGTTAAC50400.020.91018514
GCGAAAA23150.020.7388524
ATCGCTT50350.020.73083345
TCGCAAT28700.020.7233513
GCGTAAT27850.020.6073923
TTAGTTA78200.020.592958
TGCGTAA28000.020.49685722
Produced by FastQC (version 0.11.5)