FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15285099
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT428440.28029913316230404No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT294650.1927694416634135No Hit
CTCGTAACCAAACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTT184980.12101982460172485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGG87300.040.9423222
CGGATTA89650.040.33444226
ACTTTCG89200.040.2245521
GGATTAG97000.037.24272527
TTAGCCC96900.037.20666530
TTCGGAT97350.036.89389424
TCGGATT100750.035.8901825
TTTCGGA101500.035.5907623
ATGTAAG109100.033.3944139
TCTACTT109900.033.2796918
AATAACG32400.033.122982
GAGATAA114100.032.7459225
ATAACGT34100.031.8709453
GATGTAA115200.031.74578938
TAAATCA118050.031.6569659
ATAAATC118950.031.4457158
TCAAATC117350.031.4336113
AATCTAC118150.031.39788216
ATCTACT118250.031.34195517
ATTAGCC117800.030.8718629
Produced by FastQC (version 0.11.5)