FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11890810
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC152440.1281998450904522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124130.10439154271239721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA16750.028.8639712
ACGTTAA17400.027.58593813
AATAGCG21050.022.9708634
TATGCCG7500.022.2723648
CGTTAAC22050.022.24172214
ATAATAC29250.021.04782117
CCGAACG23850.020.7136443
GCACTAC30800.020.55428325
GTCCGAA32900.020.4140621
AACGGTA23800.020.1722816
GCCTAAG20950.019.91738567
TAGCGCT24150.019.73520736
GGCCTAA21800.019.6947666
CGCAAAT24450.019.63294830
CGAACGG24550.019.414314
TAATACA32100.019.28736518
CGCAATA16150.018.53008745
TGAATCG26100.018.39681442
GATCGGA9850.018.3710961
GCTAGCG8400.018.23385254
Produced by FastQC (version 0.11.5)