FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11890810
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG389830.32784141702709907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAACG11500.023.544412
TCTCGAT26050.021.9929948
TAACGTC13950.020.8969484
ATAACGT13500.020.822233
TTCTCGA28850.020.21844547
CGGATTA18850.020.0753426
ATTCCGT28850.019.98262853
CTCGATT28850.019.97909549
TAGCTCT29750.019.25472840
CTTTCGG19100.018.54051822
TTCCGTG31900.018.39944354
ACACGGA31900.017.84899316
ACTTTCG20750.017.23287421
CGGACAC32650.017.225313
TTCGGAT21950.016.92376524
GCATGGC62450.016.87837670
ACGGACA36250.016.85620118
TTAGCCC21600.016.71566630
CGGACAG37500.016.47879619
ACCCGGC36050.016.2703765
Produced by FastQC (version 0.11.5)