FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14903789
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC35050.027.96707517
TAATACA35750.027.0305418
AATAATA38350.025.56060816
TGCGTAA13600.025.30444722
CTCGCCG54150.024.98476670
GCGTAAT13950.024.6696523
GACGTTA11500.023.8790312
GATTTAT45250.023.6655835
TACGACA15050.023.0966769
TACGTCG15000.022.72620444
TATTGAA46900.022.5313249
GCACTAC43600.022.40410825
GAAAATA46300.021.24676713
TTACGAC17250.021.1585648
ACGTTAA12950.020.93689513
TTCGGCG47150.020.36195444
TCGGCGC48500.020.22754545
CCGTACG48700.020.2041744
GTCGCAA17050.020.18358212
CGCAATC17300.020.09290714
Produced by FastQC (version 0.11.5)