FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14903789
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCG33350.019.77026670
TCTCGAT22400.019.68647448
TAGGTCG10000.018.7317623
TCGTAAC22350.018.4738672
TACGTAA6700.017.67345663
CGTAGAT38250.017.6091651
CGTAACC24200.017.0575433
TTCTCGA27550.017.01293247
ATTCCGT26500.016.65216853
CTCGATT26450.016.5419949
TAGATTC46700.015.8214973
CTCGTAA28800.015.7923681
CCTAGGT14850.015.664511
CTAAATT56450.015.61985345
TAGTATA24550.015.54983641
CCTAAAT58600.015.10453744
GTAGATT48050.015.0918082
TAGCTCT28850.015.03606640
TTCCGTG30950.014.70851754
ACGTAAG8550.014.67012664
Produced by FastQC (version 0.11.5)