FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16304883
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC201980.12387700052800134No Hit
CCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT198060.12147281277639342No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG189960.116504975840673No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT164300.10076735907887226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAA27950.038.4276522
GCGTAAT28500.036.83222223
TACGTCG28450.036.55834644
CGCAATC30000.036.03238714
TTACGTC31750.032.97547543
GTCGCAA32500.032.8328712
CGGATTA32300.032.54033749
GGTCGCA33050.032.28648811
ATAATAC42650.031.94632117
TCGCAAT34400.031.92879313
CTCGCCG65650.030.64582870
AACGCCG39750.030.608531
AATAATA45250.030.49489216
TAATACA45900.029.76014518
ATCAACG35150.029.667128
TTGGTCG37800.029.3324789
GCCGCAA39150.028.6823834
CGGTACG7250.028.5615768
CTGCGTA39150.028.23326921
TGGTCGC39750.027.19416810
Produced by FastQC (version 0.11.5)