FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16304883
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAC34850.024.782632
CGTAACC36500.023.3707773
CGAATAG24700.022.7659051
CTCGTAA43550.020.4041581
TCTCGAT27800.020.10326648
TTAGCCC22200.019.84322530
CTCGATT29100.019.44554749
CGCCTAA19100.018.72101849
CTTTCGG23750.018.69401222
TTCTCGA31000.018.02615447
ATTCCGT31050.018.01294953
TAGGTCG9300.017.1516463
TTCCGTG34050.017.04150654
TAGCTCT33800.016.83208840
CGCGAAC14150.016.73625870
GCTACCG36100.016.55633570
TATAGTA40300.016.4411639
ATTCCGC39100.016.41130614
GTATAGC40400.016.23252143
CTTAGAT34600.016.1516421
Produced by FastQC (version 0.11.5)