FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14254069
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA13050.027.71659712
ATAATAC35950.025.8302117
GCACTAC38650.023.30712125
CTCGCCG43850.023.16583870
ACGTTAA15900.022.74852613
AATAATA43500.021.66676316
TAATACA44250.020.98514718
TTCGGCG34350.020.7658644
TCGGCGC35900.020.16090245
CGCAATC12450.020.11315514
TACGACA11800.019.7472579
GCGTAAT12700.019.44365123
GATTTAT53800.019.3335295
ACTACAG49300.019.18935627
CGCAATA15000.019.02232745
CGAGGCG48250.018.8323254
CGGAACG13200.018.7192348
TACGTCG12650.018.70428844
CACTACA49550.018.67157626
TGCGTAA13350.018.49695422
Produced by FastQC (version 0.11.5)