FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15015226
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210570.14023764943664518No Hit
CGCCAACATGGGCCGCGTTCGCACCAAAACCGTGAAGAAGGCGGCCCGGG210320.14007115177620372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGC97500.029.10474270
GTTGCCG18250.027.36385570
CGCCTAA25450.025.49840449
CTTTCGG21700.023.02707522
GCGGTGT28450.022.9307813
CGGTGTC29250.022.89679314
GATTAGC22700.020.94337328
TCCGCGT16600.020.9013527
CCGCCTA31300.020.84352748
CGAGCTT30550.020.6709334
ACACGCG94100.020.32503169
GGACCCG69050.020.1134091
CGCCAAC91550.019.9108751
ACTTTCG25300.019.47540521
GCGTTCG88050.019.3899915
CGGATTA25300.019.06477726
CGTTCGC90900.018.93506816
ACCCGCC74600.018.9256083
CGCTTGT19850.018.88000912
TTCGCAC91750.018.8721718
Produced by FastQC (version 0.11.5)