FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20485961
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA573220.2798111350499984No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGT482460.2355076239772203No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT409960.20011753414936206No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG317060.15476940525269964No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG316940.15471082855229493No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG297960.14544594710494665No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT282780.1380359945037482No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT277600.13550743360294398No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCT262550.12816093909385065No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT243240.1187349717203894No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC243000.11861781831957992No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA238840.11658715937221593No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA238690.11651393849671002No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC237650.11600627375986901No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA229570.11206210926595046No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA218090.10645827159389788No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG212990.10396876182669683No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT208410.10173308442791627No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA206280.1006933479957323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG39150.042.0386923
GCACTAC89950.037.55159425
GCGCGTT15500.035.66508562
CGGAACG65550.035.56920248
ATAATAC97250.035.33775717
CTCGCCG64250.035.1972870
CGAGGCG94850.034.8428254
GTTACGA47750.034.4673234
TAATACA101200.033.8555618
AGCACTA102950.033.01201224
ACTACAG104850.032.44698727
CCCCGAG104700.031.69986551
GGCGTTT105300.031.58934657
TACGACT52750.031.3978276
TTACGAC53600.030.9646935
GATTTAT120150.030.9506235
CACTACA109700.030.91767126
CGCTCGC80450.030.09668556
CTACAGA114200.030.03338828
CGTTTTG111000.030.03040759
Produced by FastQC (version 0.11.5)