FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13294786
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT169470.12747102510713598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC52450.030.23390617
GCACTAC56550.027.85875925
GCGCGTT4050.027.54022262
TAATACA58400.027.27276418
CTCGCCG39550.027.1299170
AATAATA62350.025.48920416
GATTTAT66150.025.3448735
TGTTACG9300.025.0590613
TATTGAA69200.023.9210729
ACTACAG68200.023.91596427
AGCACTA67050.023.85898224
AGATTTA67300.023.2062114
GACGTTA10350.023.1840112
GAAAATA69650.023.11743513
CGCAATA15650.022.90082445
CACTACA69050.022.8661626
ATTGAAA73500.022.00115410
CGAGGCG69350.021.88754854
TACGACT10950.020.9653826
ATACGAA17300.020.92202449
Produced by FastQC (version 0.11.5)