FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11593494
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212060.18291293375405207No Hit
CCTGGCTCACGAGGTTGGCTGGAAGTACCAGGCAGTGACAGCCACCCTGGAGGAGAAGAGGAAAGAGAAAGCCA166560.14366678414634967No Hit
CGACAAGAAAAAGCGGATGGTGGTTCCTGCTGCCCTCAAGGTCGTGCGTC155470.13410107427493384No Hit
AGAAGATCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGATTGTGGAG143700.12394882854124908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG3650.024.663393
CGCCTAA22050.023.44420849
TAGCGTA6500.022.3795172
CGCATTT18150.022.33998143
GCGGTGT23750.021.87405413
CGTAGAT43750.021.8640631
GGGTAGG13250.021.69455316
CTATTCT25400.021.40785826
GGTAGGC13850.021.00522817
TCGCAAT26100.020.84026540
TGCCTAT101250.020.46403370
TTATCGT10900.020.3302466
CTAAATT27650.019.80919845
ATTCCGT14400.019.77733453
GTCTCGC23200.019.5511576
CCGCCTA26550.019.46665648
TATTCTC28350.019.42464827
GTAGATT49300.019.3197062
TAGATTC50100.019.2814083
GCGTTCG8400.018.96529415
Produced by FastQC (version 0.11.5)