FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15273924
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT300540.19676672477877982No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261830.17142287731692263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205130.13430078609792742No Hit
CCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT176490.11554987441341205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC81400.034.46349317
GCACTAC81400.034.1239725
TAATACA86200.032.58452618
CTCGCCG38800.030.43116870
AATAATA94550.029.63364616
AGCACTA93550.029.61743524
GATTTAT99650.029.553975
CACTACA94600.029.47273426
ACTACAG95450.029.28302227
TATTGAA99750.028.8903799
GAAAATA102950.027.85740313
CGGAACG21750.027.67975448
TGTTACG11850.027.5814953
GCTAGCG17300.027.57883854
CGAGGCG97300.027.5600154
GTCCCCG102600.026.72987649
CTACAGA105250.026.35825228
CCCCGAG103500.026.13363851
AGATTTA102350.025.7167644
ATACAGC108250.025.69090320
Produced by FastQC (version 0.11.5)