FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15273924
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT240940.15774597280960675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220810.1445666483609582No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT181760.1190001992939077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGG47250.035.69556422
TTAGCCC51300.033.07859830
TAGCATA33350.032.1519139
CGGATTA53850.031.38506526
GATTAGC55950.030.33152628
ACCCGGC60900.029.7944055
TTCGGAT57100.029.53840624
ACACGGA61750.029.05526416
CGGACAC63200.028.49781413
ACGGACA65850.027.82554618
GCATGGC104650.027.82013970
GGATTAG60950.027.67284227
TAGCCCA61950.027.3923231
CGGACAG67550.027.12447219
TCGGATT64450.026.27696625
CCGGACA68950.026.2218512
TCTCGAT69750.025.8872948
CACCCGG70750.025.6942924
TCACCCG72150.025.5804713
CACGGAC72700.025.39526417
Produced by FastQC (version 0.11.5)