FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15690040
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT468880.2988392636347645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA213840.13629028351744163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAC99850.039.3974125
CGAGGCG102100.037.11738254
ATAATAC107150.037.0994317
TAATACA109800.036.04557818
ACTACAG112450.035.3535627
CCCCGAG109550.034.7676451
AGCACTA113450.034.73551624
CACTACA114900.034.41858326
GATTTAT124300.033.4708825
AATAATA120700.033.42399216
TGTTACG15500.033.411713
GTCCCCG116100.033.24471349
TATTGAA123350.033.1283769
GCGCGTT9200.032.61901562
GGCGTTT120150.031.93641357
CGTTTTG122200.031.27185259
CTACAGA127000.031.1938628
GAAAATA128200.031.00782813
TACAGAA132250.030.2182129
AGATTTA123500.029.9445234
Produced by FastQC (version 0.11.5)