FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12792302
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431790.33753893552544334No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC276640.2162550571429599No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA205100.16033079894455274No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG160160.12520029624066098No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT157880.1234179743411311No Hit
GTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTT149630.11696878325730585No Hit
CCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCC144190.11271622574263802No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA136640.1068142387507737No Hit
CTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCGTGCGG135080.10559475534583221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA39100.031.57535712
ACGTTAA41950.029.84469813
CTCGCCG34850.027.08880870
CGCAATC27200.026.97803114
TACGTCG28250.026.72679144
AAGACGT47350.026.5143210
TGCGTAA27750.026.06797222
TCGCAAT28550.025.94598813
TACGATA2300.025.70500817
GGTCGCA28700.025.56792811
TAGCGCT48400.025.36934336
AATAGCG48350.025.32284434
ATAGCGC48250.025.3038335
CGTTAAC49850.025.04528414
GCGTAAT28900.024.91042123
TGCGAAA17550.024.776696
AACGCCG31950.024.60254931
GCGAAAA18100.024.5938427
TTGGTCG31100.024.4893369
GCGCTGA51350.023.77800438
Produced by FastQC (version 0.11.5)