FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11007329
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGATCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGATTGTGGAG150020.13629101119808446No Hit
AGCGAAGAGAAGATCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGAT139020.12629766948911947No Hit
CCTGGCTCACGAGGTTGGCTGGAAGTACCAGGCAGTGACAGCCACCCTGGAGGAGAAGAGGAAAGAGAAAGCCA131480.11944768799042893No Hit
CACCACGGAAAGAGAAGGGTTCACGGGAAGAGAAGCAGAAGCCCCAGGCT111550.10134156978500415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAT33150.024.7692991
TCGTAAC15650.024.3367542
GTTATCG11950.021.7273435
CTCGTAA19700.020.7522281
TAGATTC39900.020.736823
GTAGATT39800.020.7051412
CGTAACC18950.020.278273
CGCATTT19000.020.24450743
CTAAATT34600.020.13669645
TTCGCAT18750.019.95590441
ATTCGCA19300.019.9236640
TGCCATT61950.019.73217270
CGTAATT3653.2105163E-919.2290770
TAAATTC37500.018.95256246
CCTAAAT37900.018.28916444
GTCTCGC21400.018.2804876
TAAACGT12850.018.20651470
ATGCGGT44450.017.9391346
TTATCGT13600.017.8189546
CGAATAG10500.017.8177721
Produced by FastQC (version 0.11.5)