FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17530896
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT841490.48000398838713093No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT318690.1817876279683594No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG291260.166140966211881No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT236060.13465369938878194No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG208590.11898422077228682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGGC249100.042.35776570
TAGCATA74550.040.2978679
TCACCCG172850.039.0850943
CTTTCGG71300.039.0708422
CGGATTA72150.038.46684626
CGGACAC175900.037.9859813
ACACGGA175450.037.84602416
ACCCGGC177000.037.641595
GATTAGC75350.037.06437728
CACCCGG180800.036.810724
CCGGACA181550.036.57441312
CGGACAG184650.036.33427419
ACGGACA185650.036.30756418
CCCGGAC183200.036.28297811
CCAGTAG82900.036.1052365
GCCCGGA184750.035.6400610
TTAGCCC77650.035.3419330
CACGGAC190750.035.26548817
TTCGGAT79400.035.17290524
CCTCACC200100.034.8555641
Produced by FastQC (version 0.11.5)