FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12730641
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG10800.029.2967553
ATAATAC37200.026.16411617
CGCAATA19650.025.49987645
CGGAACG21250.024.73323248
CGATTAG17750.024.2898714
ACGTTCG21150.023.8374356
CTCGCCG36200.022.99028470
GCACTAC43250.022.58551225
GTTACGA14200.022.5268944
AATAATA44150.022.51772916
GCGAAAA13950.022.4321124
TAATACA43750.022.40588218
GATTTAT49400.022.1710115
ATACGAA22800.021.83326549
TACGACT15400.021.2231086
TATTGAA49450.020.8776289
GTGCGAT24200.020.83861539
TCGATTA21200.020.6650543
TGCGAAA15150.020.6553123
GCTAGCG9350.020.48984154
Produced by FastQC (version 0.11.5)