FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13728013
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA971250.7074949593943421No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470820.34296296193775455No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG263300.19179760392126669No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193540.14098180122644113No Hit
GTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCC185790.135336410302059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCCG76400.035.41800370
CGCAATA16350.028.0763645
ATACGAA16200.027.50162949
ACGCATA4900.026.26413746
TCGGCGC74100.026.09403445
ACTCGCC79300.025.56790469
TTCGGCG77950.024.6665344
AATACGA18850.024.18365348
TACGAAT19350.023.93179950
CCGTACG81150.023.923794
TACGCCA82500.023.7787387
CGTACTA2350.023.70591750
TACGTAT3850.023.4569535
GCGTAAT15250.023.45135723
GCGTTAG4200.023.3847765
CGTACGC82900.023.3768675
CGCCGCG82950.023.31623628
TGCGTAA15300.023.14778122
GCGGGGA83800.022.96663937
GTCGCAA15350.022.61965212
Produced by FastQC (version 0.11.5)