FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13728013
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1281010.9331357713603563No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA393590.2867057308293633No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281350.20494590149353734No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225950.16459046185343792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139710.10177000852199077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT23050.024.8501948
CTCGATT23850.023.4388749
CCCGCGG65350.022.79752570
ATTCCGT24650.022.42332553
TCACGTA8550.021.8846368
TTAGCCC24250.021.8634330
TTCTCGA26600.020.87652847
TAGCTCT28400.020.14330340
TCGTAAC25800.020.0148642
CGAATAG21300.019.8628651
ACGTAGC9800.019.80103510
TACGTAT4900.019.796925
ACTTTCG27000.019.63659921
CTCAAAA123050.019.5868631
ACCGGTA15500.019.25290746
CTTTCGG27500.019.02796622
CGTAACC27900.018.7505823
TTACGTA6100.018.7414844
CGCTAGG4006.4028427E-1018.6227370
TTAGCGA4350.018.3442545
Produced by FastQC (version 0.11.5)