FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20600136
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA404820.19651326573766312No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG360430.17496486430963368No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG357100.17334837012726517No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT288340.13996994971295335No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGT280680.1362515276598174No Hit
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT279500.13567871590750663No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT267220.12971759021396753No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG253450.12303316832471398No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA237190.11514001655134705No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT234470.1138196369189019No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT231590.1124215878963129No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC227680.11052354217467301No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA211830.10282941821354964No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCT210230.10205272431211133No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA206540.10026147400191922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG48100.040.5322573
CTCGCCG67500.033.37870
GTTACGA59150.032.725134
GCGCGTT14950.030.83454962
CGGAACG63900.030.72341548
GCACTAC72650.030.7047425
ATAATAC76400.029.87750217
TACGACT65900.029.531586
TTACGAC66450.029.2347815
TGCGCGG27350.027.56585913
TAATACA83150.027.45195418
CGAGGCG80050.026.68492754
GATTTAT94450.026.3815425
TTCGGCG62950.026.19480144
AATAATA89800.025.80593916
CGCGGAC28150.025.79508815
GCGCGGA28200.025.74935314
ACTACAG88800.025.74660927
TCGGCGC63900.025.48199745
CGCTCGC81400.025.4818456
Produced by FastQC (version 0.11.5)